-Relevant antibody bound to a solid phrase (polystyrene microtiter plates)
-Heat-treated broth culture (from food) added to the well
-Unbound antigen washed away
-Enzyme labeled antibody (conjugate) is added
-Incubated
-After incubation, unbound conjugate washed away
-Specific substrate added – to show the reaction via enzymatic reactions
-Incubation at room temperature
-Reaction ceased by addition of sulfuric acid
-Results read through photometer at specific wavelength
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment